Journal: Scientific Reports
Article Title: Comparative analysis of liquid and gel platelet rich plasma from apheresis and buffy coat in wound healing
doi: 10.1038/s41598-025-30402-w
Figure Lengend Snippet: Effect of PRPs treatments on HDMEC investigating: ( A ) Schematic representation of the experimental design with HDMEC. ( B ) Proliferation. WST-1 assay evaluated after 72 h of PRPs treatment. n ≥ 3 replicates. Data are expressed as % of 10% FBS control. ( C ) Migration. Number of HDMEC cells migrated into a scratch test after 64 h of treatment with PRPs. Data are obtained by constructing a reference area and counting the number of cells migrating within the area over time. n ≥ 3 replicates. ( D ) Circularity. A cellular shape descriptor, circularity, was measured on n ≥ 15 cells/condition. Circularity is in a range between 1 and 0, where 1 identifies completely circular cells, and 0 sharp cells. ( E ) Representative phase-contrast images of HDMEC cellular shape. Images are collected after 64 h of PRPs treatment. Scale bar 100 μm. ( F ) Gene Expression levels of specific EMT and normal endothelial markers. ACTA2 (αSMA), VWF, SELE and COL1A1 expression (2 -ΔCt ) in HDMEC fibroblasts evaluated by qPCR after 72 h of treatment with PRP. Different bar colours indicate the following conditions: black for 5% FCS, grey for 0.1% FBS, red for Aphe liquid, blue for BC liquid, pink for Aphe gel and light blue for BC gel. Data are expressed as mean ± SD. Significance of data differences was established using unpaired Two-Way ANOVA test with multiple comparisons and simple t-test for the comparison between FCS 5% and FCS 0.1%. * p-value < 0.05, ** p-value < 0.01, *** p-value < 0.001.
Article Snippet: Human Dermal Microvascular Endothelial Cells cell line (HDMEC, Cat.No.C-12212PromoCell, Germany), were cultured in the Endothelial Cell Basal Medium MV (Cat.No.C-22220, PromoCell, Germany) supplemented with the Endothelial Cell Growth Medium MV Supplement Pack (Cat.No.C-39220, PromoCell, Germany) at 37 °C, 5% CO2.
Techniques: WST-1 Assay, Control, Migration, Gene Expression, Expressing, Comparison
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